Cell Biology Cover Letter

In response to your job posting for cell biology, I am including this letter and my resume for your review.

Previously, I was responsible for high quality scientific and technical leadership to a team of graduate and PhD level staff involved in the development of innovative cell culture techniques, with a focus on primary, patient-derived, disease-specific cells and complex cell culture systems.

My experience is an excellent fit for the list of requirements in this job:

• Demonstrated experience with cell/tissue culture methods, differentiation to neural lineages, morphological cell culture characterization and immunocytochemistry
• Demonstrated experience in molecular methods such as RNA sequencing, PCR, ChIP-sequencing and western blot, including sample processing and data analysis and interpretation
• Demonstrated experience in analyzing RNA-Seq datasets of neuronal lineage cells for markers of neuronal identity and function
• Working knowledge of molecular biological techniques, including molecular cloning, DNA mutagenesis, expression vector design, nucleic acid analysis, RT-PCR, genotyping, Southern blotting, protein engineering, protein purification, protein analysis, Western blotting, ELISA, mass spectroscopy, protein binding assay, and immunofluorescence assay
• Knowledge of personal computers and software to include experience with MS Office (Word, Excel, PowerPoint) and Adobe (Photoshop, Acrobat)
• Experience with cell cultures and sterile techniques, creating primary cell lines, maintenance and storage of cell lines and tissue samples
• Knowledge of light and fluorescent microscopy and familiarity with image analysis
• Experience with cell reprogramming techniques and iPSC generation

I submit this application to express my sincere interest in the cell biology position.

Previously, I was responsible for scientific guidance and technical expertise for biological assessment of lipid formulations on cross functional platform teams.

I reviewed the requirements of the job opening and I believe my candidacy is an excellent fit for this position. Some of the key requirements that I have extensive experience with include:

• Hands on experience with T-cell functional characterization assays including in vitro cytotoxicity, proliferation, and cytokine/chemokine measurements
• Strong background in cell biology, cancer biology and immunology
• Excellent verbal and written skills and integral working ethics
• Tissue culture, bioassay (cytolytic preferred), ELISA experience
• Method transfer, method qualification and method validation experience preferred
• A strong background in cell, molecular or cancer biology
• Experience in a range of cell and molecular techniques including
• Experience working with in vivo models is desirable

I would like to submit my application for the cell biology opening. Please accept this letter and the attached resume.

In the previous role, I was responsible for scientific perspectives with respect to cell and molecular biology, media development, and upstream process development as well as process scale-up required for early and late stage process development.

Please consider my experience and qualifications for this position:

• Establish internal research capabilities in Hypoxia biology to support drug discovery and small molecule characterization for pipeline development
• Establish, execute and analyze cell-based, biochemical and molecular assays critical to project objectives
• Interact as part of the Research Team with Principal Investigators and other key personnel at Contract Research Organizations (CROs) for study coordination and planning, protocol development, research report review, and project timelines
• Contribute to the writing, publishing and presentation of major scientific findings
• Proven track record of advancing Drug Discovery programs is desirable
• Experience in the independent design and execution of experiments
• Publications in peer-reviewed journals is desirable
• BA/BS in biology-related discipline

Please consider me for the cell biology opportunity. I am including my resume that lists my qualifications and experience.

In my previous role, I was responsible for scientific guidance and technical expertise for biological assessment of modified mRNA on cross functional platform teams.

Please consider my qualifications and experience:

• At least 6 months of hands-on experiences in cell culture, WB, DNA purification, and PCR
• Strong knowledge and technical skills in in vitro culture of mammalian cell lines
• Experience with 3D cell culture systems is highly valuable
• Technical skills in growing, passaging & freezing mammalian cell lines under stringent aseptic techniques
• Previous experience with cell types used in cell therapy applications
• Demonstrated leadership and management of collaborative projects across departments, functions and/or organizations
• Hands-on expertise in in cell culture of multiple cell lines and aseptic technique
• Experience with isolation, stimulation and in vitro expansion of primary T cells

I submit this application to express my sincere interest in the cell biology position.

In the previous role, I was responsible for scientific input to design of cell-mediated immunoassays (CMI) including ELISpot and/or multi-color flow cytometry) are needed for Regulated studies, both preclinical and clinical.

My experience is an excellent fit for the list of requirements in this job:

• Develop and initiate collaborative working internally and externally, providing guidance to other staff and students
• Experience with stem or progenitor cell culture, aseptic technique, and/or chromatography
• Data collection and analysis - Performs quality control, monitor data for accuracy, upload data to database/documentation systems within agreed upon timelines with supervisor
• Good interpersonal skills and communication skills to permit clear and effective exchanges of information with colleagues from within and outside of the immediate working group
• Self-motivated and demonstrated strong work ethic
• Excited to learn new skills as projects evolve and eager to contribute
• Knowledge of pediatric brain diseases
• Experience in setting up, maintaining, and troubleshooting cell and molecular biological laboratory equipment